Removal of PCR inhibitors from soil DNA by chemical flocculation
نویسندگان
چکیده
منابع مشابه
Removal of PCR inhibitors from soil DNA by chemical flocculation.
Extracting high-purity DNA directly from soil has become essential for the study of microorganisms in environmental samples. However, many soils contain compounds that inhibit enzymes involved in manipulating DNA. In this study, chemical flocculation using multivalent cations was investigated as a potential method for eliminating soil-based inhibitors during the extraction process. The addition...
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ژورنال
عنوان ژورنال: Journal of Microbiological Methods
سال: 2003
ISSN: 0167-7012
DOI: 10.1016/s0167-7012(02)00210-5